When utilizing BaseSpace Sequence Hub PrepTab, i5 sequences needs to be entered into these software within the forward orientation in all situation and for all instrument varieties.
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Which means these software program work on the idea that your i5 sequence is usually in the forward orientation and can instantly generate the reverse complement in the sequences offered for the right instrument type.
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Exclusive twin indexing provides totally exclusive, unrelated index sequences to both equally finishes of each fragment of DNA for the duration of library preparing.
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When working with IEM, i5 sequences needs to be entered into these software program while in the ahead orientation in all situation and for all instrument styles.
When evaluating index sets for color balance, the sequence noticed with the instrument have to be assessed, regardless of the orientation used for the sample sheet. With the i7 index, generally asses colour balance utilizing the "i7 bases for sample sheet" column orientation.
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